Gt(ROSA)26Sor^{tm1.1(CAG-mKate*)Bbr}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(CAG-mKate*)Bbr}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Benoit G Bruneau
• MGI ID: MGI:6790613
• Synonyms: Rosa^nKmB
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(CAG-mKate*)Bbr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:232545
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The targeting construct targetted into the locus a nuclear localized mKateV5 (nlsmKateV5) followed by a rabbit globin polyA sequence cloned between ROX recombination sites downstream of a CAGGS promoter. An FRT-pGK Neo-FRT cassette was also inserted in the construct, but it was removed by breeding mice to Flpe expressing mice. It is a fluorescent Dre-reporter allele in which upon execution of Roxed stop cassette, the fluorescent mKate can be visualized in the cells. (J:232545)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1060 strains or lines available

References

• Original: J:232545 Devine WP, et al., Early patterning and specification of cardiac progenitors in gastrulating mesoderm. Elife. 2014;3
• All: 1 reference(s)