Gt(ROSA)26Sor^{tm87(CAG-gltI/GFP*)Hze}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm87(CAG-gltI/GFP*)Hze}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 87, Hongkui Zeng
• MGI ID: MGI:6790279
• Synonyms: Ai87(RCL-iGluSnFR)
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm87(CAG-gltI/GFP*)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:219930
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The Rosa-CAG targeting vector CAG-LSL-iGluSnFR-WPRE-bGHpA was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. The targeting vector was designed with a floxed-STOP cassette, the iGluSnFr coding sequence (details below), a WPRE sequence (to enhance mRNA stability), a BGH polyA. The iGluSnFr fusion gene is constructed from the Escherichia coli gltI gene and circularly permutated green fluorescent protein (cpGFP). Specifically, iGluSnFr has a 21 aa IgG K-leader sequence, the gltI aa 1-253, the LILV linker, the cpGFP (aa 148-239, an 18 bp flexible linker, and aa 1-147), the L2NP linker, the gltI aa 254-279, an 11 aa c-Myc epitope and a 49 aa PDGR sequence (PDGR aa 513-561). iGluSnFr is an extremely fast and sensitive glutamate indicator with a large signal-to-noise ratio, and kinetics appropriate for in vivo imaging. It is a Cre-dependent reporter with a glutamate indicator function. (J:219930)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1060 strains or lines available

References

• Original: J:219930 Madisen L, et al., Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron. 2015 Mar 4;85(5):942-58
• All: 2 reference(s)