Gt(ROSA)26Sor^{tm72(CAG-hop/EGFP)Hze}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm72(CAG-hop/EGFP)Hze}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 72, Hongkui Zeng
• MGI ID: MGI:6790278
• Synonyms: Ai72(RCL-VSFPB)
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm72(CAG-hop/EGFP)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:219930
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The Rosa-CAG targeting vector CAG-LSL-VSFP-Butterfly1.2-WPREbGHpA was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. The targeting vector was designed with a floxed-STOP cassette (stop codons in all three reading frames linked to synthetic pA-hGHpA-PGKpA), the voltage-sensitive fluorescent protein Butterfly 1.2 coding sequence, a WPRE sequence (to enhance mRNA stability), a BGH polyA. It is a Cre-dependent reporter with a voltage indicator function. (J:219930)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1095 strains or lines available

References

• Original: J:219930 Madisen L, et al., Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron. 2015 Mar 4;85(5):942-58
• All: 1 reference(s)