Hint1^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Hint1^{tm1b(EUCOMM)Wtsi}
• Name: histidine triad nucleotide binding protein 1; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:6782387
• Gene: Hint1 Location: Chr11:54757209-54761327 bp, + strand Genetic Position: Chr11, 32.13 cM
• Alliance: Hint1^{tm1b(EUCOMM)Wtsi} page
• IMPC: Hint1 gene page

Mutation origin

• Mutant Cell Line: EPD0326_6_G01
• Germline Transmission: Earliest citation of germline transmission: J:311513
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 54760244 of Chromosome 11 upstream of exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the exon 3 at position 54761593. Exons 2 and 3 are thus flanked by loxP sites. Cre mediated recombination without prior flp recombination created a reporter knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:311513)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hint1 Mutation: 24 strains or lines available

References

• Original: J:311513 Sato M, et al., The lncRNA Caren antagonizes heart failure by inactivating DNA damage response and activating mitochondrial biogenesis. Nat Commun. 2021 May 5;12(1):2529
• All: 1 reference(s)