Cacna1s^tm1.1Ics
Targeted Allele Detail

Summary

• Symbol: Cacna1s^tm1.1Ics
• Name: calcium channel, voltage-dependent, L type, alpha 1S subunit; targeted mutation 1.1, Mouse Clinical Institute
• MGI ID: MGI:6781961
• Synonyms: ncDHPR
• Gene: Cacna1s Location: Chr1:135980549-136047268 bp, + strand Genetic Position: Chr1, 59.55 cM, cytoband F
• Alliance: Cacna1s^tm1.1Ics page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:252980
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Single point mutation
• Mutation details: A mutation was engineered in exon 13 to change asparagine codon 617 (AAC) to an aspartic acid codon (GAC)(p.N617D) and a loxP site, an FRT site flanked protamine-cre cassette, a neomycin resistance gene cassette and a second loxP site were inserted in opposite transcriptional orientation into intron 12. The floxed cre plus neo cassette pair was self-excised in male germline through the protamine promotor-driven expression of Cre. The mutation in this allele creates a non-Ca2+-conducting form of the encoded peptide. (J:252980)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cacna1s Mutation: 117 strains or lines available

References

• Original: J:252980 Dayal A, et al., The Ca2+ influx through the mammalian skeletal muscle dihydropyridine receptor is irrelevant for muscle performance. Nat Commun. 2017 Sep 07;8(1):475
• All: 4 reference(s)