Dcn^tm1Pjro
Targeted Allele Detail

Summary

• Symbol: Dcn^tm1Pjro
• Name: decorin; targeted mutation 1, Peter J Roughley
• MGI ID: MGI:6781928
• Synonyms: decorin KI
• Gene: Dcn Location: Chr10:97315471-97354005 bp, + strand Genetic Position: Chr10, 50.27 cM
• Alliance: Dcn^tm1Pjro page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:311974
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Using homologous recombination in ES cells, the targeting construct was designed to generate a T-to-G mutation in exon 2, changing serine codon 34 to alanine (p.S34A). A neomycin resistance gene cassette was inserted into the intron 2. The serine 34 to alanine substitution at the dermatan sulfate (DS) attachment site of decorin produces only the decorin core protein with its N-linked oligosaccharides and is unable to produce the proteoglycan form of decorin. (J:311974)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dcn Mutation: 29 strains or lines available

References

• Original: J:311974 Moffatt P, et al., Absence of the dermatan sulfate chain of decorin does not affect mouse development. J Negat Results Biomed. 2017 Apr 17;16(1):7
• All: 1 reference(s)