Casp8^em1Dgre
Endonuclease-mediated Allele Detail

Summary

• Symbol: Casp8^em1Dgre
• Name: caspase 8; endonuclease-mediated mutation 1, Douglas R Green
• MGI ID: MGI:6781656
• Synonyms: Casp8^D387A
• Gene: Casp8 Location: Chr1:58834553-58886663 bp, + strand Genetic Position: Chr1, 29.19 cM, cytoband B
• Alliance: Casp8^em1Dgre page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Aspartic acid codon 387 (GAT) was targeted for change to an alanine codon (GCG)(p.D387A) with an sgRNA (targeting TAATACGACTCACTATAGGACAGAACCACACTTTAGAAGGTTTTAGAGCTAGAAATAGCA) and an ssODN template (CCAAGATCTTTTTCATTCAGGCTTGCCAAGGAAGTAACTTCCAGAAAGGAGTGCCTGATGAGGCAGGCTTCGAGCAACAGAACCACACTTTGGAGGTTGCGAGCTCATCTCACAAGAACTATATTCCGGATGAGGCAGACTTTCTGCTGGGAATGGCTACGGTGAAGAACTGCGTTTCCTACCGAGATCCTGTGAATGGA) using CRISPR/Cas9 technology. The mutation creates an uncleavable form of the encoded peptide. (J:296046)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Casp8 Mutation: 45 strains or lines available

References

• Original: J:296046 Tummers B, et al., Caspase-8-Dependent Inflammatory Responses Are Controlled by Its Adaptor, FADD, and Necroptosis. Immunity. 2020 Jun 16;52(6):994-1006.e8
• All: 3 reference(s)