Adamts7^em1Macb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Adamts7^em1Macb
• Name: ADAM metallopeptidase with thrombospondin type 1 motif 7; endonuclease-mediated mutation 1, Bryan MacDonald
• MGI ID: MGI:6779988
• Synonyms: Adamts7 E373Q
• Gene: Adamts7 Location: Chr9:90041420-90082155 bp, + strand Genetic Position: Chr9, 47.46 cM
• Alliance: Adamts7^em1Macb page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: CRISPR/cas9 genome editing is used to create an amino acid substitution at position 373 (glutamate to glutamine, GAG to CAG, E373Q) and a silent mutation in the Cas9 PAM site (Ala371A - GCC to GCT - to prevent additional editing). The mutation preserves the helical structure of the HExxH motif but prevents the generation of a reactive nucleophile at the zinc metalloprotease site. (J:309184)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Adamts7 Mutation: 66 strains or lines available

References

• Original: J:309184 Mizoguchi T, et al., Coronary Disease Association With ADAMTS7 Is Due to Protease Activity. Circ Res. 2021 Aug 6;129(4):458-470
• All: 1 reference(s)