Adcyap1r1^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Adcyap1r1^{tm1c(KOMP)Wtsi}
• Name: adenylate cyclase activating polypeptide 1 receptor 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6771734
• Gene: Adcyap1r1 Location: Chr6:55428963-55478436 bp, + strand Genetic Position: Chr6, 27.38 cM, cytoband B3
• Alliance: Adcyap1r1^{tm1c(KOMP)Wtsi} page
• IMPC: Adcyap1r1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:311566
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 55454675 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 55455376. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:173534, J:311566)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Adcyap1r1 Mutation: 35 strains or lines available

References

• Original: J:311566 Van C, et al., Targeted deletion of PAC1 receptors in retinal neurons enhances neuron loss and axonopathy in a model of multiple sclerosis and optic neuritis. Neurobiol Dis. 2021 Oct 2;160:105524
• All: 5 reference(s)