Sh3gl2^tm1Gpt
Targeted Allele Detail

Summary

• Symbol: Sh3gl2^tm1Gpt
• Name: SH3-domain GRB2-like 2; targeted mutation 1, GemPharmatech Co., Ltd
• MGI ID: MGI:6771625
• Gene: Sh3gl2 Location: Chr4:85123663-85307617 bp, + strand Genetic Position: Chr4, 40.23 cM
• Alliance: Sh3gl2^tm1Gpt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:311551
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 85272993 of Chromosome 4 upstream of the exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 85274075. Exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. (J:311551)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sh3gl2 Mutation: 37 strains or lines available

References

• Original: J:311551 Yang Y, et al., Endophilin A1 Promotes Actin Polymerization in Dendritic Spines Required for Synaptic Potentiation. Front Mol Neurosci. 2018;11:177
• All: 2 reference(s)