Scn2a^tm1aNarl
Targeted Allele Detail

Summary

• Symbol: Scn2a^tm1aNarl
• Name: sodium channel, voltage-gated, type II, alpha; targeted mutation 1a, National Laboratory Animal Center
• MGI ID: MGI:6771479
• Synonyms: Scn2a^gt
• Gene: Scn2a Location: Chr2:65451115-65597791 bp, + strand Genetic Position: Chr2, 38.61 cM
• Alliance: Scn2a^tm1aNarl page
• IMPC: Scn2a gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:311530
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: IMPC

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted upstream of the critical exon. The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon. The critical exons are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:311530)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Scn2a Mutation: 90 strains or lines available

References

• Original: J:311530 Zhang J, et al., Severe deficiency of the voltage-gated sodium channel NaV1.2 elevates neuronal excitability in adult mice. Cell Rep. 2021 Aug 3;36(5):109495
• All: 3 reference(s)