Tlk1^tm1.1Gle
Targeted Allele Detail

Summary

• Symbol: Tlk1^tm1.1Gle
• Name: tousled-like kinase 1; targeted mutation 1.1, Gustavo Leone
• MGI ID: MGI:6765360
• Synonyms: Tlk1^F
• Gene: Tlk1 Location: Chr2:70542751-70656072 bp, - strand Genetic Position: Chr2, 41.9 cM
• Alliance: Tlk1^tm1.1Gle page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:255302
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6 x 129

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: A targeting vector from IKMC Project 71713 was used to generate this allele. The L1L2_Bact_P cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 9 and 10. The critical exons 9 and 10 are thus flanked by loxP sites. Flp-mediated recombination deleted the lacZ and neomycin resistance gene leaving exons 9 and 10 floxed. (J:255302)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tlk1 Mutation: 207 strains or lines available

References

• Original: J:255302 Liu H, et al., Discovery of Stromal Regulatory Networks that Suppress Ras-Sensitized Epithelial Cell Proliferation. Dev Cell. 2017 May 22;41(4):392-407.e6
• All: 1 reference(s)