Sympk^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Sympk^{tm1c(EUCOMM)Wtsi}
• Name: symplekin; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6765313
• Synonyms: Sympk^Flox
• Gene: Sympk Location: Chr7:18758321-18788542 bp, + strand Genetic Position: Chr7, 9.46 cM
• Alliance: Sympk^{tm1c(EUCOMM)Wtsi} page

Mutation origin

• Mutant Cell Line: EPD0069_2_H09
• Germline Transmission: Earliest citation of germline transmission: J:255302
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 18763987 of Chromosome 7 upstream of the critical exons 4 and 5 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exons 4 and 5 at position 18764906. The critical exons 4 and 5 are thus flanked by loxP sites. Flp-mediated recombination removed the lacZ/neomycin sequence and created a "conditional ready" (floxed) allele. (J:255302)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sympk Mutation: 44 strains or lines available

References

• Original: J:255302 Liu H, et al., Discovery of Stromal Regulatory Networks that Suppress Ras-Sensitized Epithelial Cell Proliferation. Dev Cell. 2017 May 22;41(4):392-407.e6
• All: 2 reference(s)