Gt(ROSA)26Sor^{tm1(CAG-Bgeo,-MAPT/mTurquoise,-MAPT/tdTomato,-Gnas*)Kmov}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-Bgeo,-MAPT/mTurquoise,-MAPT/tdTomato,-Gnas*)Kmov}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Kiavash Movahedi
• MGI ID: MGI:6764576
• Synonyms: Gt(ROSA)26Sor^{tm1(CAG-Bgeo,-MAPT/mCerulean,-MAPT/tdTomato,-Gnas*)Kmov}, RoMo-dnGs Knock-In
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-Bgeo,-MAPT/mTurquoise,-MAPT/tdTomato,-Gnas*)Kmov} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:310193
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, Dominant negative, Inserted expressed sequence, RMCE-ready, Reporter)
• Mutation: Insertion
• Mutation details: A targeting construct consisting of CMV enhancer sequence, a beta actin promoter, a floxed beta-geo stop cassette, loxN, Kozak-bovine tau/mTurquoise-WPRE-pA, lox2272, loxN, Kozak-bovine tau/tdTomato-IRES-dominant-negative rat Gnas mutant-WPRE-pA, lox2272 was inserted via homologous recombination. The allele allows stochastic Cre/lox recombination for functional mosaic analysis utilizing two distinct subpopulation after cre mediated recombination. (J:310193)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1083 strains or lines available

References

• Original: J:310193 Movahedi K, et al., RoMo: An efficient strategy for functional mosaic analysis via stochastic Cre recombination and gene targeting in the ROSA26 locus. Biotechnol Bioeng. 2018 Jul;115(7):1778-1792
• All: 1 reference(s)