Gt(ROSA)26Sor^{tm3(CAG-YAP1*,-lacZ)Jmao}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm3(CAG-YAP1*,-lacZ)Jmao}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 3, Junhao Mao
• MGI ID: MGI:6763056
• Synonyms: nls-YAP5SA^KI, R26-YAP5SA-NLS
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm3(CAG-YAP1*,-lacZ)Jmao} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:245870
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Constitutively active, Epitope tag, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: The following elements were inserted into the locus: the CAG promoter, a loxP site, a neomycin resistance gene cassette, a STOP cassette (four poly(A) signal sequences), a second loxP site, a mutated human yes-associated protein 1 cDNA (where all five serine codons were changed to alanine to create a constitutively active form of the encoded peptide) with 5' in-frame FLAG tag and NLS coding sequence, an IRES, and the lacZ beta galactosidase reporter gene. Only after cre-mediated removal of the neo-STOP cassette will this locus express the YAP1 and lacZ genes. (J:245870, J:283491)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1084 strains or lines available

References

• Original: J:245870 Cotton JL, et al., YAP/TAZ and Hedgehog Coordinate Growth and Patterning in Gastrointestinal Mesenchyme. Dev Cell. 2017 Oct 09;43(1):35-47.e4
• All: 5 reference(s)