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Ace2em1Guru
Endonuclease-mediated Allele Detail
Summary
Symbol: Ace2em1Guru
Name: angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; endonuclease-mediated mutation 1, Channabasavaiah Gurumurthy
MGI ID: MGI:6763023
Synonyms: mAce2KI-31K,82M,353K
Gene: Ace2  Location: ChrX:162922338-162971414 bp, + strand  Genetic Position: ChrX, 76.12 cM, cytoband F5
Alliance: Ace2em1Guru page
Mutation
origin
Strain of Origin:  C57BL/6J
Mutation
description
Allele Type:    Endonuclease-mediated (Humanized sequence)
Mutation:    Nucleotide substitutions
 
Mutation detailsCRISPR/cas9 endonuclease-mediated genome editing is used to insert 5 amino acid substitutions. The amino acids 30, 31, 34 in exon 2 were changed from asparagine to glutamic acid (N30D), asparagine to lysine (N31K), and glutamine to aspartic acid (Q34D) respectively, amino acid 82 in exon 3 was changed from serine to methionine (S82M), and the amino acid 353 in exon 9 was changed from histidine to lysine (H353K). The mutations are designed to completely humanize the mouse Ace2 coding sequence in a region important for binding to the SARS-CoV-2 spike protein. (J:298750)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Ace2 Mutation:  36 strains or lines available
References
Original:  J:298750 Gurumurthy CB, et al., Genetically modified mouse models to help fight COVID-19. Nat Protoc. 2020 Dec;15(12):3777-3787
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/15/2022
MGI 6.22
The Jackson Laboratory