Rgs3^tm1.1Qlu
Targeted Allele Detail

Summary

• Symbol: Rgs3^tm1.1Qlu
• Name: regulator of G-protein signaling 3; targeted mutation 1.1, Qiang Lu
• MGI ID: MGI:6762184
• Gene: Rgs3 Location: Chr4:62478079-62621256 bp, + strand Genetic Position: Chr4, 33.19 cM
• Alliance: Rgs3^tm1.1Qlu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:299414
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: By homologous recombination in ES cells, a loxP site was inserted into the mouse Rgs3 gene at intron1 and another loxP followed by a phospho-glycerate kinase-1-neo-bpA cassette flanked by FRT was inserted into intron 5. The resulted allele has Rgs3 gene exon 2 through exon 5 coding for PDZ domain flanked by loxP followed by a FRT flanked neo selection cassette. Cre-mediated recombination in germ cells resulted in the deletion of exon 2 through exon 5. The loss of Rgs3 mRNA was confirmed by RTPCR and the loss of full-length protein was also confirmed with western blot. (J:299414)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rgs3 Mutation: 45 strains or lines available

References

• Original: J:299414 Qiu R, et al., Essential role of PDZ-RGS3 in the maintenance of neural progenitor cells. Stem Cells. 2010 Sep;28(9):1602-10
• All: 2 reference(s)