Zbtb20^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Zbtb20^{tm1c(EUCOMM)Hmgu}
• Name: zinc finger and BTB domain containing 20; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6761852
• Synonyms: Zbtb20 floxed
• Gene: Zbtb20 Location: Chr16:42728008-43462981 bp, + strand Genetic Position: Chr16, 28.44 cM, cytoband B4
• Alliance: Zbtb20^{tm1c(EUCOMM)Hmgu} page
• IMPC: Zbtb20 gene page

Mutation origin

• Mutant Cell Line: HEPD0822_1_A11
• Germline Transmission: Earliest citation of germline transmission: J:308922
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 43429246 of Chromosome 16 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 43431419. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml Cell line HEPD0822_1_A11 was successfully used to make chimeric mice. Germline transmission was accomplished. J:308922 (J:308922)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zbtb20 Mutation: 56 strains or lines available

References

• Original: J:308922 Medeiros de Araujo JA, et al., ZBTB20 is crucial for the specification of a subset of callosal projection neurons and astrocytes in the mammalian neocortex. Development. 2021 Aug 15;148(16):dev196642
• All: 1 reference(s)