Itpr1^em1Akino
Endonuclease-mediated Allele Detail

Summary

• Symbol: Itpr1^em1Akino
• Name: inositol 1,4,5-trisphosphate receptor 1; endonuclease-mediated mutation 1, Akira Kinoshita
• MGI ID: MGI:6761816
• Synonyms: Itpr1^delta7bp
• Gene: Itpr1 Location: Chr6:108190057-108528070 bp, + strand Genetic Position: Chr6, 49.74 cM
• Alliance: Itpr1^em1Akino page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology was used to design guide RNAs that disrupt exons 59 and 62 of mouse Itpr1. Three founder mice with a deletion in exon 59 at a high mosaic rate died soon after birth for unknown reasons. Another mouse line with a seven-base pair deletion (c.8238_8244delAGCCGGC) in the last exon of Itpr1 which resulted in a frameshift and readthrough mutation was established and used for the study. (J:308935)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Itpr1 Mutation: 185 strains or lines available

References

• Original: J:308935 Kinoshita A, et al., Itpr1 regulates the formation of anterior eye segment tissues derived from neural crest cells. Development. 2021 Aug 15;148(16):dev188755
• All: 1 reference(s)