Fgf21^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Fgf21^{tm1c(EUCOMM)Hmgu}
• Name: fibroblast growth factor 21; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6761770
• Synonyms: Fgf21^LoxP
• Gene: Fgf21 Location: Chr7:45263314-45264914 bp, - strand Genetic Position: Chr7, 29.38 cM, cytoband B2
• Alliance: Fgf21^{tm1c(EUCOMM)Hmgu} page
• IMPC: Fgf21 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:234346
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 45263308 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 45264113. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:234346)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fgf21 Mutation: 21 strains or lines available

References

• Original: J:234346 Vernia S, et al., Fibroblast Growth Factor 21 Mediates Glycemic Regulation by Hepatic JNK. Cell Rep. 2016 Mar 15;14(10):2273-80
• All: 3 reference(s)