Gt(ROSA)26Sor^{tm5(CAG-EYFP*,-mCherry*,-EGFP*,-Maml1*,-mCerulean*,-Notch1*)Ben}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm5(CAG-EYFP*,-mCherry*,-EGFP*,-Maml1*,-mCerulean*,-Notch1*)Ben}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 5, Rui Benedito
• MGI ID: MGI:6759816
• Synonyms: Gt(Rosa)26Sor^{tm1(iChr-Notch-Mosaic)}, iChr-Notch-Mosaic
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm5(CAG-EYFP*,-mCherry*,-EGFP*,-Maml1*,-mCerulean*,-Notch1*)Ben} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:252386
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm5(CAG-EYFP*,-mCherry*,-EGFP*,-Maml1*,-mCerulean*,-Notch1*)Ben} expresses 2 genes
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Maml1 (MGI:1890504)	sequence coding for amino-acids 13-74 of mouse peptide is downstream in-frame with T2A sequence which is downstream in-frame with EGFP gene
  mouse	Notch1 (MGI:97363)	sequence coding for the intra-cellular and PEST domains is downstream in-frame with T2A sequence which is downstream in-frame with mCerulean gene

• Mutation details: This allele has three chromatin/nuclear-targeted fluorescent protein sequences uniquely flanked with pairs of incompatible lox sites (in the same direction), all inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. The targeting vector is designed with (from 5' to 3') two copies of chicken beta-globin HS4 insulator element (2xINS; increase downstream gene expression and minimize regulatory interference), a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a loxN site, a lox2272 site, a loxP site, the N-PhiMut gene (non-cytotoxic/non-fluorescent Phialidium yellow fluorescent protein variant PhiYFP with nuclear localization signal), SV40 polyA signal sequence, a second loxN site, a full consensus KOZAK sequence, the His-H2B-Cherry gene (monomeric DsRed fluorescent protein variant mCherry with N-terminal 6xHis affinity tag and 125 amino acid human histone 2B chromatin/nucleus tag sequence), in-frame Thosea asigna virus 2A cleavage site (T2A; mediates ribosomal skipping), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance mRNA transcript stability), SV40 polyA signal sequence, a second lox2272 site, a full consensus KOZAK sequence, the H2B-EGFP-V5 gene (enhanced green fluorescent protein with 127 amino acid human histone 2B chromatin/nucleus tag plus C-terminal paramyxovirus V5 tag sequence), in-frame T2A sequence, in-frame sequence for a dominant-negative form of mouse Maml1 (DN-Maml1 coding for amino-acids 13-74), WPRE sequence, SV40 polyA signal sequence, a second loxP site, a full consensus KOZAK sequence, the HA-H2B-mCerulean gene (monomeric enhanced cyan fluorescent protein variant mCerulean with N-terminal hemagglutinin tag and 125 amino acid human histone 2B chromatin/nucleus tag sequence), in-frame T2A sequence, in-frame mouse Notch1 intracellular domain (NICD) and PEST domain sequence (NICD-PEST), WPRE sequence, SV40 polyA signal sequence, an FRT site flanked neomycin resistance gene cassette, and a second 2xINS sequence. (J:252386)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1095 strains or lines available

References

• Original: J:252386 Pontes-Quero S, et al., Dual ifgMosaic: A Versatile Method for Multispectral and Combinatorial Mosaic Gene-Function Analysis. Cell. 2017 Aug 10;170(4):800-814.e18
• All: 2 reference(s)