Foxp3^{tm12.1(Hbegf/EGFP)Ayr}
Targeted Allele Detail

Summary

• Symbol: Foxp3^{tm12.1(Hbegf/EGFP)Ayr}
• Name: forkhead box P3; targeted mutation 12.1, Alexander Y Rudensky
• MGI ID: MGI:6758784
• Synonyms: Foxp3^fl-DTR, Foxp3^{tm12.1(DTR/EGFP)Ayr}
• Gene: Foxp3 Location: ChrX:7445915-7461482 bp, + strand Genetic Position: ChrX, 3.41 cM
• Alliance: Foxp3^{tm12.1(Hbegf/EGFP)Ayr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:248943
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Foxp3^{tm12.1(Hbegf/EGFP)Ayr} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  Not Specified	Hbegf

• Mutation details: A loxP site; encephalomyocarditis virus IRES; diptheria toxin receptor (DTR) enhanced green fluorescent protein (eGFP) fusion protein; a triple SV40 polyA (STOP); a second loxP site; encephalomyocarditis virus IRES; Thy1.1; followed by a frt site-flanked PGK-Neomycin resistance gene (NEO)-BGHpA cassette (loxP-IRES-DTReGFP-STOP-loxP-IRES-Thy1.1-frt-NEO-BGHpA-frt) was inserted into the Foxp3 3' UTR via homologous recombination in ES cells. The mice were bred to FLPeR mice to excise the NEO cassette. (J:248943)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Foxp3 Mutation: 61 strains or lines available

References

• Original: J:248943 Levine AG, et al., Stability and function of regulatory T cells expressing the transcription factor T-bet. Nature. 2017 Jun 15;546(7658):421-425
• All: 1 reference(s)