Gt(ROSA)26Sor^{tm3(CAG-Hoxa5,-GFP)Fmr}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm3(CAG-Hoxa5,-GFP)Fmr}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 3, Filippo M Rijli
• MGI ID: MGI:6758674
• Synonyms: ROSA^{CAG-lsl-Hoxa5-KI}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm3(CAG-Hoxa5,-GFP)Fmr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:306314
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm3(CAG-Hoxa5,-GFP)Fmr} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Hoxa5 (MGI:96177)

• Mutation details: The following elements were inserted into the ROSA locus: the CAG promoter, a loxP site flanked STOP cassette, sequence for an N-terminal 3xFlag tag in-frame with a codon-optimized mouse Hoxa5 cDNA, poly(A) signal sequences, IRES sequence, the GFP fluorescent marker gene, a WPRE element, poly(A) signal sequences and a neomycin resistance gene cassette. Only after cre-mediated removal of the neo-STOP cassette will this allele express the Hoxa5 and GFP genes. (J:306314)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1098 strains or lines available

References

• Original: J:306314 Maheshwari U, et al., Postmitotic Hoxa5 Expression Specifies Pontine Neuron Positional Identity and Input Connectivity of Cortical Afferent Subsets. Cell Rep. 2020 Jun 16;31(11):107767
• All: 2 reference(s)