Col1a1^{tm1(tetO-T6B/YFP)Aven}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(tetO-T6B/YFP)Aven}
• Name: collagen, type I, alpha 1; targeted mutation 1, Andrea Ventura
• MGI ID: MGI:6758668
• Synonyms: T6B
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(tetO-T6B/YFP)Aven} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:314127
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Humanized sequence, Inducible, Inserted expressed sequence, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: The targeting vector expressing the FLAG tag-HA tag-T6B-YFP transcript under control of TRE and CMV minimal promoter, was generated from a modified version of the pgk-ATG-FRT plasmid, in which the region of pgk-ATG-FRT comprised between the EcoRI site and the PciI site was substituted with the rabbit beta-globin polyadenylation signal (RBG pA). The FLAG-HA-T6B-YFP DNA insert was generated by PCR using the plasmid pIRES-Neo-FLAG-HA-T6B-YFP as a template. Next, the modified pgk-ATG-FRT, was cut with NcoI, filled-in to produce blunt ends, dephosphorylated and ligated to the PCR-generated CMV-TRE-FLAG-HA-T6B-YFP DNA fragment. T6B is a short Tnrc6b-derived peptide (amino acid position 599-683) that competes with TNRC6 protein binding. (J:314127)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 160 strains or lines available

References

• Original: J:314127 La Rocca G, et al., Inducible and reversible inhibition of miRNA-mediated gene repression in vivo. Elife. 2021 Aug 31;10:e70948
• All: 1 reference(s)