Prkn^tm1Tchi
Targeted Allele Detail

Summary

• Symbol: Prkn^tm1Tchi
• Name: parkin RBR E3 ubiquitin protein ligase; targeted mutation 1, Tomoki Chiba
• MGI ID: MGI:6758222
• Synonyms: Parkin^-
• Gene: Prkn Location: Chr17:11059271-12282248 bp, + strand Genetic Position: Chr17, 7.8 cM, cytoband A3.2-A3.3
• Alliance: Prkn^tm1Tchi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:309429
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Most of exon 2 was replaced in-frame by the coding sequence of tau (Mapt) and green fluorescent protein (GFP), followed by translation and transcription termination sequences and the MC1-neomycin cassette. Western blot analysis confirmed the absence of protein in the brain. RT-PCR confirmed the presence of GFP transcripts, however the parkin-tau-GFP fusion protein is not clearly detectable by Western blot or immunohistochemical analysis. (J:309429)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prkn Mutation: 55 strains or lines available

References

• Original: J:309429 Sato S, et al., Decline of striatal dopamine release in parkin-deficient mice shown by ex vivo autoradiography. J Neurosci Res. 2006 Nov 1;84(6):1350-7
• All: 3 reference(s)