Ankrd24^em1Pgg
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ankrd24^em1Pgg
• Name: ankyrin repeat domain 24; endonuclease-mediated mutation 1, Peter G Gillespie
• MGI ID: MGI:6755469
• Gene: Ankrd24 Location: Chr10:81464374-81483444 bp, + strand Genetic Position: Chr10, 39.72 cM, cytoband C1
• Alliance: Ankrd24^em1Pgg page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR-targeting made a large deletion between exons 6 and 17 of 6,214-bp. The indel starts with an imperfect insertion of 7 bp (T/GCTTCC) where the initial T, part of the intronic sequence, is the first base variation from the wild-type sequence. The remaining 6 bp of the insert match the first bases of exon 6, afterward, then a deletion of 6,207-bp removes exons 7-16 and the first 65-bp of exon 17. This deletion results in a frameshift mutation in which the first 6 bp of exon 6, position 327 from the start codon, joins directly to the last 1,312-bp of exon 17 and an early stop at codon 114. (J:321477)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ankrd24 Mutation: 47 strains or lines available

References

• Original: J:321477 Krey JF, et al., ANKRD24 organizes TRIOBP to reinforce stereocilia insertion points. J Cell Biol. 2022 Apr 4;221(4):e202109134
• All: 1 reference(s)