Gt(ROSA)26Sor^tm1.1Etz
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^tm1.1Etz
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Elena Tzouanacou
• MGI ID: MGI:6752560
• Synonyms: R26nlaacZ1.1 exc
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^tm1.1Etz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:278250
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: A vector, which contains (5' to 3') a splice acceptor, floxed neo/stop cassette and sequence encoding mutated nuclear localized lacZ with intragenic duplication corresponding to nucleotides 870-1987 after the ATG such that a shift in codon phase introduces a TGA STOP codon at nucleotide 1993 was inserted into the locus via homologous recombination. The parental Gt(ROSA)26Sor^tm1Etz was crossed to cre deleter to remove the floxed neo/stop cassette. The duplication in the lacZ sequence truncated b-galactosidase molecule making the lacZ inactive, except in small fraction of cells undergone infrequent spontaneous random intragenic homologous recombination restoring the functional lacZ sequence. (J:278250)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1058 strains or lines available

References

• Original: J:278250 Tzouanacou E, et al., Redefining the progression of lineage segregations during mammalian embryogenesis by clonal analysis. Dev Cell. 2009 Sep;17(3):365-76
• All: 1 reference(s)