Igh^{tm2.1(Myc)Janz}
Targeted Allele Detail

Summary

• Symbol: Igh^{tm2.1(Myc)Janz}
• Name: immunoglobulin heavy chain complex; targeted mutation 2.1, Siegfried Janz
• MGI ID: MGI:6741072
• Synonyms: c-myc-KICmu, iMyc^deltaEmu
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:308700
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Epitope tag, Inserted expressed sequence)
• Mutations: Insertion, Intergenic deletion
• Igh^{tm2.1(Myc)Janz} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Myc (MGI:97250)	mouse cDNA with 3' His6 tag

• Mutation details: Heavy-chain enhancer Emu, downstream of the J gene segment, was replaced with a construct consisting of a 1.5 kb segment of genomic 5' flanking sequence from the mouse Myc gene with the P1/P2 promoters, the first noncoding exon, a mouse Myc intron-less cDNA clone with an in-frame 6x histidine tag sequence on the 3' end, a short segment of the 3' UTR including the major polyadenylation signal, and a loxP site flanked neomycin resistance gene cassette, all in opposite transcriptional orientation compared to the host gene. The neo cassette was removed through subsequent cre-mediated recombination. (J:308700)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 47 strains or lines available

References

• Original: J:308700 Duncan K, et al., (18)F-FDG-PET/CT imaging in an IL-6- and MYC-driven mouse model of human multiple myeloma affords objective evaluation of plasma cell tumor progression and therapeutic response to the proteasome inhibitor ixazomib. Blood Cancer J. 2013 Nov 29;3:e165
• All: 5 reference(s)