Gt(ROSA)26Sor^{tm2.1(CAG-HIST1H2BB/EGFP)Zjh}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2.1(CAG-HIST1H2BB/EGFP)Zjh}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2.1, Z Josh Huang
• MGI ID: MGI:6741027
• Synonyms: H2B-GFP
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2.1(CAG-HIST1H2BB/EGFP)Zjh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:308660
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector is designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP-flanked STOP cassette, a FRT-flanked STOP cassette, an H2B-EGFP fusion protein (coding sequence for the human HIST1H2BB gene [histone 1 H2bb] followed C-terminally by an enhanced green fluorescent protein sequence), and a Neo-polyA cassette. The entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. Cre mediated recombination removed the loxP-flanked STOP cassette. This alle was generated by crossing mouse with parental Gt(ROSA)26Sor^{tm2(CAG-HIST1H2BB/EGFP)Zjh} to cre-deleter mouse. It is an flp reporter. (J:308660)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1095 strains or lines available

References

• Original: J:308660 Liu MZ, et al., Synaptic control of spinal GRPR(+) neurons by local and long-range inhibitory inputs. Proc Natl Acad Sci U S A. 2019 Dec 5
• All: 1 reference(s)