Gt(ROSA)26Sor^{tm5.1(CAG-lacZ)Dym}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm5.1(CAG-lacZ)Dym}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 5.1, Susan Dymecki
• MGI ID: MGI:6740237
• Synonyms: RC-FSF-LacZ
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm5.1(CAG-lacZ)Dym} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:290856
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: This insertion contains (in order) a CAGGS promoter, FRT-flanked neo cassette with 2 poly-adenylation sequences, floxed EGFP followed by 5 poly-adenylation sequences and a lacZ gene with a nuclear localization sequence. Parental Gt(ROSA)26Sor^{tm5(CAG-EGFP,-lacZ)Dym} was crossed to cre deleter mice to remove EGFP-STOP cassette. Nuclear-lacZ expression results from FLP- mediated recombination. (J:290856)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1060 strains or lines available

References

• Original: J:290856 Poulin JF, et al., PRISM: A Progenitor-Restricted Intersectional Fate Mapping Approach Redefines Forebrain Lineages. Dev Cell. 2020 Jun 22;53(6):740-753.e3
• All: 1 reference(s)