Gt(ROSA)26Sor^{tm11.1(Cas9)Npa}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm11.1(Cas9)Npa}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 11.1, Novartis Pharma AG
• MGI ID: MGI:6724105
• Synonyms: Rosa26-SpCas9, Rosa26 ^tm7<1-5CNpa
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm11.1(Cas9)Npa} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:293207
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Endonuclease, Epitope tag)
• Mutation: Insertion
• Mutation details: By homologous recombination in the C57BL/6J ES cells, a SA-3xFLAG-NLS-SpCas9-NLS-polyA-loxP-HSVtk-Neo-loxP cassette was inserted into the Gt(ROSA)26Sor locus. Targeted ES cells were used for blastocyst injection to obtain chimeric mice. The loxP-HSVtk-Neo-loxP cassette was removed by mating with cre deleter mice. (J:293207)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1042 strains or lines available

References

• Original: J:293207 Planas-Paz L, et al., YAP, but Not RSPO-LGR4/5, Signaling in Biliary Epithelial Cells Promotes a Ductular Reaction in Response to Liver Injury. Cell Stem Cell. 2019 Jul 3;25(1):39-53.e10
• All: 1 reference(s)