Gt(ROSA)26Sor^{tm1.1(CAG-CHRM3*/mCherry)Stak}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(CAG-CHRM3*/mCherry)Stak}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Takeshi Sakurai
• MGI ID: MGI:6724031
• Synonyms: Rosa26^dreaddm3
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(CAG-CHRM3*/mCherry)Stak} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:294111
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The targeting construct consisting of a CAG promoter, a loxp, 2xpA, FRT-flanked Pgk-neo selection cassette, STOP cassette, loxp, followed by a hM3Dq/mCherry fusion protein, pA signal all inserted into the Gt(ROSA)26Sor locus was used to generate the mouse line by homologous recombination in the C57BL/6N ES cells. The pgk-Neo cassette was removed with FLP66. CAG promoter drives expression of hM3Dq-mCherry only after Cre-mediated excision of the floxed stopper element. (J:294111)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1062 strains or lines available

References

• Original: J:294111 Takahashi TM, et al., A discrete neuronal circuit induces a hibernation-like state in rodents. Nature. 2020 Jul;583(7814):109-114
• All: 2 reference(s)