Gt(ROSA)26Sor^{tm1(CAG-TGFB1*)Lba}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-TGFB1*)Lba}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Laurent Bartholin
• MGI ID: MGI:6723946
• Synonyms: ^FSFTGFb^CA
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-TGFB1*)Lba} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:294598
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: A construct comprising the following elements was inserted into the Gt(ROSA)26Sor locus: CAG promoter, a FRT site, a neomycin resistance gene cassette, a STOP cassette, a FRT site, the constitutionally active mutant form of simian TGFB1 cDNA containing the two point mutations C223S/C225S (TGF^CA), IRES sequence, the eYFP fluorescent reporter gene, and bGH poly(A) sequence. The inserted TGFCA gene and the reporter gene will only be expressed after removal of the neo-STOP cassette through flp-mediated recombination. (J:294598)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1095 strains or lines available

References

• Original: J:294598 Cardot-Ruffino V, et al., Generation of a conditional Flpo/FRT mouse model expressing constitutively active TGFbeta in fibroblasts. Sci Rep. 2020 Mar 3;10(1):3880
• All: 1 reference(s)