Tg(Pvalb-GFP/Ldlr)3Tkane
Transgene Detail

Summary

• Symbol: Tg(Pvalb-GFP/Ldlr)3Tkane
• Name: transgene insertion 3, Takeshi Kaneko
• MGI ID: MGI:6721437
• Synonyms: PV/myrGFP-LDLRct
• Transgene: Tg(Pvalb-GFP/Ldlr)3Tkane Location: unknown
• Alliance: Tg(Pvalb-GFP/Ldlr)3Tkane page

Transgene origin

• Strain of Origin: B6D2F1

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The transgene consists of a fusion protein myristoylation/palmitoylation site-attached GFP (myrGFP) and DNAs encoding C-terminal cytoplasmic domains (CT) of mouse low-density lipoprotein receptor (Ldlr; amino acid residues 813862), LDLRct inserted into the start codon located in the exon 2 of the Pvalb gene so that it is under the control of the mouse Pvalb promoter. The modified BAC transgene construct DNA was purified and linearized then injected into pronuclei of B6D2F1 mouse zygotes. Five founder lines 1-5 were generated. Line 3 with the co-expression rate of GFP and PV immunoreactivities was highest and used for the study. (J:187049)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:187049 Kameda H, et al., Parvalbumin-producing cortical interneurons receive inhibitory inputs on proximal portions and cortical excitatory inputs on distal dendrites. Eur J Neurosci. 2012 Mar;35(6):838-54
• All: 3 reference(s)