Exoc1^{tm1b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Exoc1^{tm1b(EUCOMM)Hmgu}
• Name: exocyst complex component 1; targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6721434
• Gene: Exoc1 Location: Chr5:76677158-76718141 bp, + strand Genetic Position: Chr5, 41.28 cM, cytoband E1
• Alliance: Exoc1^{tm1b(EUCOMM)Hmgu} page
• IMPC: Exoc1 gene page

Mutation origin

• Mutant Cell Line: HEPD0703_7_C02
• Germline Transmission: Earliest citation of germline transmission: J:104881
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 76685016 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 76685993. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:104881)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Exoc1 Mutation: 53 strains or lines available

References

• Original: J:104881 The RIKEN BioResource Center, Information obtained from The RIKEN BioResource Center. Unpublished. 2006-2013
• All: 2 reference(s)