Pgk1^{tm1(CAG-EGFP*)Koba}
Targeted Allele Detail

Summary

• Symbol: Pgk1^{tm1(CAG-EGFP*)Koba}
• Name: phosphoglycerate kinase 1; targeted mutation 1, Kazuto Kobayashi
• MGI ID: MGI:6721047
• Synonyms: Pgk1-eGFP, Pgk1^{tm1(CAG-eGFP-NLS,PGK-Neo)Koba}
• Gene: Pgk1 Location: ChrX:105230706-105247305 bp, + strand Genetic Position: ChrX, 47.36 cM, cytoband C-D
• Alliance: Pgk1^{tm1(CAG-EGFP*)Koba} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:240352
• Parent Cell Line: EGR-G101 (ES Cell)
• Strain of Origin: C57BL/6NSlc-Tg(CAG-EGFP,Acr-EGFP)2Osb

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change, Reporter)
• Mutation: Insertion
• Mutation details: The targeting construct introduced into intron 11, an FRT site, the inverted cassette containing the CAG promoter driving expression of the EGFP reporter gene with a nuclear localization sequence, a floxed neomycin resistance cassette, and an FRT site. (J:240352)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pgk1 Mutation: 23 strains or lines available

References

• Original: J:240352 Kobayashi S, et al., Live imaging of X chromosome reactivation dynamics in early mouse development can discriminate naive from primed pluripotent stem cells. Development. 2016 Aug 15;143(16):2958-64
• All: 4 reference(s)