Card19^tm1Lex
Targeted Allele Detail

Summary

• Symbol: Card19^tm1Lex
• Name: caspase recruitment domain family, member 19; targeted mutation 1, Lexicon Pharmaceuticals
• MGI ID: MGI:6720298
• Synonyms: Card19^lxcn
• Gene: Card19 Location: Chr13:49356426-49369471 bp, - strand Genetic Position: Chr13, 25.15 cM
• Alliance: Card19^tm1Lex page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:307276
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The targeting vector is designed to replace exons 3-6 (encoding the C-terminal half of the CARD and the entire CARD19 C-terminus) with a betageo-puro cassette. Absence of full-length protein expression was confirmed by western blot analysis of homozygous CD8+ T cells. Subsequent RNAseq and western blotting revealed hypomorphic expression of the adjacent Ninj1 gene. (J:307276)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Card19 Mutation: 11 strains or lines available

References

• Original: J:307276 Rios KE, et al., CARD19, the protein formerly known as BinCARD, is a mitochondrial protein that does not regulate Bcl10-dependent NF-kappaB activation after TCR engagement. Cell Immunol. 2020 Oct;356:104179
• All: 3 reference(s)