Gt(ROSA)26Sor^{tm6.1(CAG-ZsGreen1)Hze}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm6.1(CAG-ZsGreen1)Hze}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 6.1, Hongkui Zeng
• MGI ID: MGI:6720233
• Synonyms: R26^Z10
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm6.1(CAG-ZsGreen1)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:155793
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The parental Gt(ROSA)26Sor^{tm6(CAG-ZsGreen1)Hze} strain containing a pair of FRT sites flanking the reporter, in addition to the flox-stop cassette placed between the CAG promoter and ZsGreen was mutated to eliminate the downstream FRT site with Crispr-CAS9aided targeting. The microinjection of the guide RNA and CAS9 constructs into fertilized embryos generated the modified alleles, named R26Z10, was established and used in the study. (J:297537)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1060 strains or lines available

References

• Original: J:297537 Wang Q, et al., A mosaic analysis system with Cre or Tomato expression in the mouse. Proc Natl Acad Sci U S A. 2020 Nov 10;117(45):28212-28220
• All: 2 reference(s)