Agpat3^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Agpat3^{tm1c(EUCOMM)Wtsi}
• Name: 1-acylglycerol-3-phosphate O-acyltransferase 3; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6719345
• Gene: Agpat3 Location: Chr10:78105012-78188323 bp, - strand Genetic Position: Chr10, 39.72 cM
• Alliance: Agpat3^{tm1c(EUCOMM)Wtsi} page
• IMPC: Agpat3 gene page

Mutation origin

• Mutant Cell Line: EPD0080_1_D08
• Germline Transmission: Earliest citation of germline transmission: J:306929
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_st1
• Mutation details: The L1L2_st1 cassette was inserted at position 78120593 of Chromosome 10 upstream of exon 4 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 78121459. Exon 4 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:306929)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Agpat3 Mutation: 22 strains or lines available

References

• Original: J:306929 Hishikawa D, et al., Hepatic Levels of DHA-Containing Phospholipids Instruct SREBP1-Mediated Synthesis and Systemic Delivery of Polyunsaturated Fatty Acids. iScience. 2020 Aug 22;23(9):101495
• All: 3 reference(s)