Gt(ROSA)26Sor^{tm1(CAG-Yap1*S112A,-EGFP)Rha}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-Yap1*S112A,-EGFP)Rha}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Ralf H Adams
• MGI ID: MGI:6719207
• Synonyms: Rosa26-Yap1^S112A, Yap1^S112A, Yap-KI
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-Yap1*S112A,-EGFP)Rha} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:306935
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Constitutively active, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(CAG-Yap1*S112A,-EGFP)Rha} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Yap1 (MGI:103262)	Yap1 contains the constitutively activating mutation S112A

• Mutation details: A vector consisting of CAG, loxP-flanked neomycin and STOP cassette, a Yap1 cDNA containing a serine to alanine substitution at amino acid 112 (S112A), FRT flanked IRES-EGFP, and pA was inserted into the locus. The S112A mutation results in a constitutively active Yap1. Expression of the constitutively active product is only achieved following cre mediated recombination. (J:306935)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1084 strains or lines available

References

• Original: J:306935 Sivaraj KK, et al., YAP1 and TAZ negatively control bone angiogenesis by limiting hypoxia-inducible factor signaling in endothelial cells. Elife. 2020 Jan 20;9:e50770
• All: 1 reference(s)