Gt(ROSA)26Sor^{tm2.1(CAG-rtTS)Gev}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2.1(CAG-rtTS)Gev}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2.1, Gerard Evan
• MGI ID: MGI:6719201
• Synonyms: Gt(ROSA)26Sor^{tm2.1(CAG-LSL-rtTS)Gev}, Rosa26^CAG-rtTS
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2.1(CAG-rtTS)Gev} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:255811
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Inducible, No functional change)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: Overlap extension PCR was used to clone the tetracycline responsive domain from rtTA*M2 (Clontech, pTet-on Advanced) to the KRAB-AB silencing domain of the Kid-1 protein (SDKid-1) (Clontech, pTet-tTS), creating the chimeric rtTS protein (tetracycline-controlled trans-silencer). The SA70b pROSA-CAGGS-attP50/B53-hygro-NLSLacZ vector was obtained from ARTEMIS Pharmaceuticals. A fragment containing the CAGGS promoter and an intron was removed from SA70b and cloned into a modified pROSA26 plasmid48, creating the pROSA26CAG-PAS vector. The rtTS cDNA above was cloned into pBigT, and then into pROSA26CAG-PAS to create the final Rosa26 CAG-LSL-rtTS targeting vector. Cre-mediated recombination removed the lox-stop-lox cassette. (J:255811)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1058 strains or lines available

References

• Original: J:255811 Gamper I, et al., Determination of the physiological and pathological roles of E2F3 in adult tissues. Sci Rep. 2017 Aug 30;7(1):9932
• All: 1 reference(s)