E2f3^tm1.1Gev
Targeted Allele Detail

Summary

• Symbol: E2f3^tm1.1Gev
• Name: E2F transcription factor 3; targeted mutation 1.1, Gerard Evan
• MGI ID: MGI:6719199
• Synonyms: E2f3^{tm1.1(TRE)Gev}, E2f3^TRE
• Gene: E2f3 Location: Chr13:30090558-30170046 bp, - strand Genetic Position: Chr13, 13.09 cM, cytoband A3.3
• Alliance: E2f3^tm1.1Gev page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:255811
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Inducible, No functional change)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: A vector containing 15 kb of the endogenous genomic sequence. The tetracycline response element (TRE) element was isolated from pTre2 (Clontech) and then cloned 500bp upstream of the translation start site along with a loxP flanked neomycin resistance cassette. No endogenous gene sequences were removed or altered at the insertion site, although silent mutations were incorporated into the first exons of E2f3a and E2f3b. Finally, a DTA cassette was added to this vector to allow negative selection in mouse embryonic stem cells. Cre-mediated recombination removed the conditional neomycin resistance cassette. (J:255811)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any E2f3 Mutation: 32 strains or lines available

References

• Original: J:255811 Gamper I, et al., Determination of the physiological and pathological roles of E2F3 in adult tissues. Sci Rep. 2017 Aug 30;7(1):9932
• All: 1 reference(s)