Ascl2^tm1.1Shiv
Targeted Allele Detail

Summary

• Symbol: Ascl2^tm1.1Shiv
• Name: achaete-scute family bHLH transcription factor 2; targeted mutation 1.1, Ramesh A Shivdasani
• MGI ID: MGI:6719079
• Synonyms: Ascl2^Dfci
• Gene: Ascl2 Location: Chr7:142520566-142523001 bp, - strand Genetic Position: Chr7, 88.08 cM
• Alliance: Ascl2^tm1.1Shiv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:307135
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Reporter)
• Mutation: Insertion
• Mutation details: The coding region containing all 3 exons was replaced with a loxP-flanked Ascl2 cDNA with a 5' triple-FLAG epitope and a 3' mCherry cDNA separated from Ascl2 by a P2A cleavage sequence. An frt-flanked neomycin selection cassette was also inserted before the second loxP site and removed via Flp-mediated recombination. (J:307135)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ascl2 Mutation: 13 strains or lines available

References

• Original: J:307135 Murata K, et al., Ascl2-Dependent Cell Dedifferentiation Drives Regeneration of Ablated Intestinal Stem Cells. Cell Stem Cell. 2020 Mar 5;26(3):377-390.e6
• All: 1 reference(s)