Kras^em2Jqmn
Endonuclease-mediated Allele Detail

Summary

• Symbol: Kras^em2Jqmn
• Name: Kirsten rat sarcoma viral oncogene homolog; endonuclease-mediated mutation 2, Patrick Jacquemin
• MGI ID: MGI:6718984
• Synonyms: Kras^Cit-G12D
• Gene: Kras Location: Chr6:145162425-145195965 bp, - strand Genetic Position: Chr6, 77.37 cM, cytoband G2
• Alliance: Kras^em2Jqmn page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Reporter)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: CRISPR/Cas9 genome editing is used to insert the Citrine cDNA with a linker sequence at the ATG start codon and to replace the site-directed mutagenized sequence for the oncogenic Kras protein in which the glycine at position 12 has been substituted to aspartic acid in the Kras locus so that the Citrine and oncogenic Kras G12D coding sequences are in the same reading frame. The construct replaced the LSLKrasG12D locus in frame with the KrasG12D gene in the male pronucleus of LSL-KrasG12D/+ mouse zygotes. (J:306509)

Tumor Data

List all tumor models in MMHCdb carrying Kras^em2Jqmn

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kras Mutation: 76 strains or lines available

References

• Original: J:306509 Assi M, et al., Dynamic Regulation of Expression of KRAS and Its Effectors Determines the Ability to Initiate Tumorigenesis in Pancreatic Acinar Cells. Cancer Res. 2021 May 15;81(10):2679-2689
• All: 1 reference(s)