Kras^em1Jqmn
Endonuclease-mediated Allele Detail

Summary

• Symbol: Kras^em1Jqmn
• Name: Kras proto-oncogene, GTPase; endonuclease-mediated mutation 1, Patrick Jacquemin
• MGI ID: MGI:6718983
• Synonyms: Kras^Cit
• Gene: Kras Location: Chr6:145162425-145195965 bp, - strand Genetic Position: Chr6, 77.37 cM, cytoband G2
• Alliance: Kras^em1Jqmn page

Mutation origin

• Strain of Origin: B6D2F2

Mutation description

• Allele Type: Endonuclease-mediated (Reporter)
• Mutation: Insertion
• Mutation details: CRISPR/Cas9 genome editing is used to insert the Citrine cDNA with a linker sequence at the ATG start codon in the Kras locus so that the Citrine and Kras coding sequences are in the same reading frame. (J:306509)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kras Mutation: 69 strains or lines available

References

• Original: J:306509 Assi M, et al., Dynamic Regulation of Expression of KRAS and Its Effectors Determines the Ability to Initiate Tumorigenesis in Pancreatic Acinar Cells. Cancer Res. 2021 May 15;81(10):2679-2689
• All: 3 reference(s)