Cryba1^em1Dbsa
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cryba1^em1Dbsa
• Name: crystallin, beta A1; endonuclease-mediated mutation 1, Debasish Sinha
• MGI ID: MGI:6718443
• Synonyms: betaA3-KO
• Gene: Cryba1 Location: Chr11:77609441-77616109 bp, - strand Genetic Position: Chr11, 46.74 cM, cytoband B-C1
• Alliance: Cryba1^em1Dbsa page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Modified isoform(s))
• Mutation: Single point mutation
• Mutation details: CRISPR/Cas9 technology generated a single nucleotide replacement of A to G in the first start codon (ATG to GTG) resulting in a knockdown of the betaA3 isoform. A silent mutation (ACC to ACG) was also introduced to prevent the binding and re-cutting of the sequence by gRNA after homology-directed repair. Western blot analysis confirmed the absence of the betaA3 isoform but also upregulation of the betaA1 isoform level is astrocytes. (J:306517)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cryba1 Mutation: 9 strains or lines available

References

• Original: J:306517 Ghosh S, et al., betaA1-crystallin regulates glucose metabolism and mitochondrial function in mouse retinal astrocytes by modulating PTP1B activity. Commun Biol. 2021 Feb 24;4(1):248
• All: 1 reference(s)