Il11^em2Tsak
Endonuclease-mediated Allele Detail

Summary

• Symbol: Il11^em2Tsak
• Name: interleukin 11; endonuclease-mediated mutation 2, Tetsushi Sakuma
• MGI ID: MGI:6715993
• Gene: Il11 Location: Chr7:4775372-4785858 bp, - strand Genetic Position: Chr7, 2.76 cM
• Alliance: Il11^em2Tsak page

Mutation origin

• Strain of Origin: Not Applicable

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutations: Intragenic deletion, Single point mutation
• Mutation details: Exon 3 was targeted with sgRNAs using CRISPR/Cas9 technology by micro-injecting a plasmid coding for two gRNAs and a Fok1-dCas9 fusion gene (restriction enzyme FokI and nuclease-deficient Cas9) into fertilized oocyte pronuclei. In founder line 11, this resulted in a 19 bp deletion and a point mutation (or a 20 bp deletion and 1 bp insertion or duplication). (J:273448)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Il11 Mutation: 21 strains or lines available

References

• Original: J:273448 Deguchi Y, et al., Generation of and characterization of anti-IL-11 antibodies using newly established Il11-deficient mice. Biochem Biophys Res Commun. 2018 Oct 28;505(2):453-459
• All: 3 reference(s)