Il11^em#Tsak
Endonuclease-mediated Allele Detail

Summary

• Symbol: Il11^em#Tsak
• Name: interleukin 11; endonuclease-mediated mutation, Tetsushi Sakuma
• MGI ID: MGI:6715980
• Gene: Il11 Location: Chr7:4775372-4785858 bp, - strand Genetic Position: Chr7, 2.76 cM
• Alliance: Il11^em#Tsak page

Mutation origin

• Strain of Origin: Not Applicable

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Not Specified
• Mutation details: Exon 3 was targeted with sgRNAs using CRISPR/Cas9 technology by micro-injecting a plasmid coding for two gRNAs and a Fok1-dCas9 fusion gene (restriction enzyme FokI and nuclease-deficient Cas9) into fertilized oocyte pronuclei. This resulted in a variety of deleterious deletions, insertions and substitutions among founder mice. (J:281516)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Il11 Mutation: 21 strains or lines available

References

• Original: J:281516 Nakagawa Y, et al., Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes. BMC Biotechnol. 2015 May 22;15:33
• All: 1 reference(s)