Eomes^tm2.1Sjar
Targeted Allele Detail

Summary

• Symbol: Eomes^tm2.1Sjar
• Name: eomesodermin; targeted mutation 2.1, Sebastian J Arnold
• MGI ID: MGI:6712744
• Synonyms: Eomes^mTnG, Eomes^{mTomatoH2B:GFP}
• Gene: Eomes Location: Chr9:118307259-118315176 bp, + strand Genetic Position: Chr9, 70.21 cM, cytoband F3
• Alliance: Eomes^tm2.1Sjar page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:243419
• Parent Cell Line: CCE916 (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The targeting construct replaced about 500 bp of the coding sequence in exon 1 with a membrane-localized tdTomato reporter gene (mTomato), a T2A sequence, nuclear-localized GFP (H2B:GFP), and polyadenylation sequence (bGH PolyA). Cre-mediated recombination removed the floxed neomycin selection cassette introduced downstream of the inserted sequence. (J:243419)

Expression

In Mice Carrying this Mutation:

24 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Eomes Mutation: 41 strains or lines available

References

• Original: J:243419 Probst S, et al., A dual-fluorescence reporter in the Eomes locus for live imaging and medium-term lineage tracing. Genesis. 2017 Aug;55(8)
• All: 4 reference(s)