Mesp1^tm1.1Sjar
Targeted Allele Detail

Summary

• Symbol: Mesp1^tm1.1Sjar
• Name: mesoderm posterior 1; targeted mutation 1.1, Sebastian J Arnold
• MGI ID: MGI:6712742
• Synonyms: Mesp1^mVenus
• Gene: Mesp1 Location: Chr7:79441989-79443338 bp, - strand Genetic Position: Chr7, 45.16 cM
• Alliance: Mesp1^tm1.1Sjar page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:301247
• Parent Cell Line: CCE916 (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Epitope tag, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The targeting construct introduced replaced the first 337 bp of the coding sequence in exon 1 a membrane-targeted Venus reporter gene (myr-Venus), a T2A sequence, the coding sequence of the endogenous gene, three FLAG epitope tags, and polyadenylation sequence (bGH PolyA). Cre-mediated recombination removed the floxed neomycin selection cassette introduced downstream of the inserted sequence. (J:301247)

Expression

In Mice Carrying this Mutation:

41 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mesp1 Mutation: 17 strains or lines available

References

• Original: J:301247 Probst S, et al., Spatiotemporal sequence of mesoderm and endoderm lineage segregation during mouse gastrulation. Development. 2021 Jan 7;148(1):dev193789
• All: 1 reference(s)